Title: Adresování kapek ATPS v mikrofluidních čipech pomocí elektrického pole
Electric field driven addressing of ATPS droplets in microfluidic chips
Authors: Vobecká, Lucie
Khafizova, Elvira
Stragier, Thomas
Slouka, Zdeněk
Přibyl, Michal
Citation: VOBECKÁ, L., KHAFIZOVA, E., STRAGIER, T., SLOUKA, Z., PŘIBYL, M. Electric field driven addressing of ATPS droplets in microfluidic chips. Microfluidics and Nanofluidics, 2017, roč. 21, č. 3, s. 1-12. ISSN 1613-4982.
Issue Date: 2017
Publisher: Springer Verlag
Document type: článek
URI: http://hdl.handle.net/11025/29248
ISSN: 1613-4982
Keywords: adresování;dvoufázové vodné systémy;elektroforéza;mikrofluidika;kapky
Keywords in different language: addressing;aqueous two-phase systems;electrophoresis;microfluidics;droplets
Abstract: Referujeme o možnosti ovlivňovat pohyb kapek (adresování kapek) pomocí stejnosměrného elektrického pole v systému dvoufázového vodného roztoku (ATPS). Byly zkoumány tři systémy ATPS typu polyetylenglykol/sůl, jmenovitě polyetylenglykol/fosfát, polyetylenglykol/síran a polyetylenglykol/uhličitan. Série pokusů s adresováním a slučováním kapek v mikrofluidním čipu ukázaly, že stejnosměrné elektrické pole může být užitečným nástrojem pro chytrou manipulaci kapek v systémech polyetylenglykol/sůl.
Abstract in different language: The possibility of controlled droplet motion (droplet addressing) mediated by DC electric field in aqueous two-phase systems (ATPS) is here reported for the first time. Three ATPS of polyethylene glycol (PEG)/salt type, namely PEG/phosphate, EG/sulphate, and PEG/carbonate, were selected for this study. We observed fast motion of salty droplets dispersed in PEG continuous phase induced by electric field of relative low strength. Hence, three fluidic systems with separated electrode chambers for the evaluation of electrophoretic mobilities and for addressing experiments were fabricated. Electrophoretic mobilities of salty droplets always exceeded the value of 1 × 10−7 m2V−1s−1, which is about by one magnitude higher value than those typically measured in water–oil droplet systems. The electrophoretic mobilities in systems with free surface are the same or even smaller than in closed microfluidic structures, which is accounted mainly to the fact that a significant part of salty droplets is exposed to air and does not contribute to droplet forcing. Series of addressing and merging experiments in a microfluidic chip shows that DC electric field can be used as a powerful tool for smart manipulation of droplets in microfluidic systems with PEG/salt ATPS.
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