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dc.contributor.authorKostelanská, Marie
dc.contributor.authorFreisleben, Jaroslav
dc.contributor.authorBačkovská Hanusová, Zdeňka
dc.contributor.authorMoško, Tibor
dc.contributor.authorVik, Robert
dc.contributor.authorMoravcová, Daniela
dc.contributor.authorHamáček, Aleš
dc.contributor.authorMosinger, Jiří
dc.contributor.authorHolada, Karel
dc.date.accessioned2019-10-28T11:00:11Z-
dc.date.available2019-10-28T11:00:11Z-
dc.date.issued2019
dc.identifier.citationKOSTELANSKÁ, M., FREISLEBEN, J., BAČKOVSKÁ HANUSOVÁ, Z., MOŠKO, T., VIK, R., MORAVCOVÁ, D., HAMÁČEK, A., MOSINGER, J., HOLADA, K. Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen. Journal of Biophotonics, 2019, roč. 12, č. 8, s. 1-13. ISSN 1864-063X.en
dc.identifier.issn1864-063X
dc.identifier.uri2-s2.0-85065342147
dc.identifier.urihttp://hdl.handle.net/11025/35696
dc.format13 s.cs
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherWileyen
dc.relation.ispartofseriesJournal of Biophotonicsen
dc.rightsPlný text je přístupný v rámci univerzity přihlášeným uživatelům.cs
dc.rights© Wileyen
dc.titleOptimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygenen
dc.typečlánekcs
dc.typearticleen
dc.rights.accessrestrictedAccessen
dc.type.versionpublishedVersionen
dc.description.abstract-translatedPrion disorders are fatal neurodegenerative diseases caused by the autocatalytic conversion of a natively occurring prion protein (PrPC) into its misfolded infectious form (PrPTSE). The proven resistance of PrPTSE to common disinfection procedures increases the risk of prion transmission in medical settings. Herein, we present the effective photodynamic inactivation (PDI) of prions by disulfonated hydroxyaluminum phthalocyanine (AlPcOH(SO3)2) utilizing two custom‐built red light sources. The treatment eliminates PrPTSE signal in infectious mouse brain homogenate with efficiency that depends on light intensity but has a low effect on the overall protein content. Importantly, singlet oxygen (O2(1Δg)) is the only species significantly photogenerated by AlPcOH(SO3)2, and it is responsible for the PDI of prions. More intensive light conditions show not only higher O2(1Δg) production but also decreases in AlPcOH(SO3)2 photostability. Our findings suggest that PDI by AlPcOH(SO3)2‐generated O2(1Δg) represents a promising approach for prion inactivation that may be useful in future decontamination strategies for delicate medical tools.en
dc.subject.translateddecontaminationen
dc.subject.translatedphotodynamic inactivationen
dc.subject.translatedphthalocyaninesen
dc.subject.translatedprionsen
dc.subject.translatedsinglet oxygenen
dc.identifier.doi10.1002/jbio.201800430
dc.type.statusPeer-revieweden
dc.identifier.document-number480614900020
dc.identifier.obd43926398
dc.project.IDLO1607/RICE-NETESIS - nové technologie a koncepce pro inteligentní průmyslové systémy (NETESIS)cs
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