Title:	SPIN enables high throughput species identification of archaeological bone by proteomics
Authors:	Rüther, Patrick Leopold Husic, Immanuel Mirnes Bangsgaard, Pernille Gregersen, Kristian Murphy Pantmann, Pernille Carvalho, Milena Godinho, Ricardo Miguel Friedl, Lukáš Cascalheira, João Taurozzi, Alberto John Schjellerup Jørkov, Marie Louise Benedetti, Michael M. Haws, Jonathan Bicho, Nuno Welker, Frido Cappellini, Enrico Olsen, Jesper Velgaard
Citation:	RÜTHER, PL. HUSIC, IM. BANGSGAARD, P. GREGERSEN, KM. PANTMANN, P. CARVALHO, M. GODINHO, RM. FRIEDL, L. CASCALHEIRA, J. TAUROZZI, AJ. SCHJELLERUP JØRKOV, ML. BENEDETTI, MM. HAWS, J. BICHO, N. WELKER, F. CAPPELLINI, E. OLSEN, JV. SPIN enables high throughput species identification of archaeological bone by proteomics. Nature Communications, 2022, roč. 13, č. 1, s. 1-14. ISSN: 2041-1723
Issue Date:	2022
Publisher:	Nature Research
Document type:	článek article
URI:	2-s2.0-85129351300 http://hdl.handle.net/11025/49887
ISSN:	2041-1723
Keywords in different language:	SPIN;proteomics;species identification;archeological bone
Abstract in different language:	Species determination based on genetic evidence is an indispensable tool in archaeology, forensics, ecology, and food authentication. Most available analytical approaches involve compromises with regard to the number of detectable species, high cost due to low throughput, or a labor-intensive manual process. Here, we introduce “Species by Proteome INvestigation” (SPIN), a shotgun proteomics workflow for analyzing archaeological bone capable of querying over 150 mammalian species by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Rapid peptide chromatography and data-independent acquisition (DIA) with throughput of 200 samples per day reduce expensive MS time, whereas streamlined sample preparation and automated data interpretation save labor costs. We confirm the successful classification of known reference bones, including domestic species and great apes, beyond the taxonomic resolution of the conventional peptide mass finger- printing (PMF)-based Zooarchaeology by Mass Spectrometry (ZooMS) method. In a blinded study of degraded Iron-Age material from Scandinavia, SPIN produces reproducible results between replicates, which are consistent with morphological analysis. Finally, we demon- strate the high throughput capabilities of the method in a high-degradation context by analyzing more than two hundred Middle and Upper Palaeolithic bones from Southern Eur- opean sites with late Neanderthal occupation. While this initial study is focused on modern and archaeological mammalian bone, SPIN will be open and expandable to other biological tissues and taxa.
Rights:	© authors
Appears in Collections:	Články / Articles (KSA) OBD

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